Nucleotide BLAST allows one to input nucleotide sequences and compare these against other nucleotides. |
Standard nucleotide-nucleotide BLAST [blastn] Takes nucleotides sequences in FASTA format, GenBank Accession numbers or GI numbers and compares them against the NCBI nucleotide databases. |
Protein BLAST allows one to input protein sequences and compare these against other protein sequences. |
Standard protein-protein BLAST [blastp] Takes protein sequences in FASTA format, GenBank Accession numbers or GI numbers and compares them against the NCBI protein databases. |
Translated BLAST Searches These searches translate either query sequences or databases from nucleotides to proteins so that protein - nucleotide sequences can be performed. |
Nucleotide query - Protein db [blastx] Converts a nucleotide query sequence into protein sequences in all 6 reading frames. The translated protein products are then compared against the NCBI protein databases. |
Protein query - Translated db [tblastn] Takes a protein query sequence and compares it against an NCBI nucleotide database which has been translated in all six reading frames. |
Nucleotide query - Translated db [tblastx] Converts a nucleotide query sequence into protein sequences in all 6 reading frames and then compares this to an NCBI nucleotide database which has been translated in all six reading frames. |